Publications

Cengiz Goekeri, Kerstin A.K. Linke, Karen Hoffmann, Elena Lopez-Rodriguez, Vladimir Gluhovic, Anne Voß, Sandra Kunder, Andreas Zappe, Sara Timm, Alina Nettesheim, Sebastian M.K. Schickinger, Christian M. Zobel, Kevin Pagel, Achim D Gruber, Matthias Ochs, Martin Witzenrath, Geraldine Nouailles. Am J Res Cell Mol Biol 2024: in press To

Vladimir Gluhovic, Sara Timm, Wolfgang M. Kuebler, Elena Lopez-Rodriguez, Matthias Ochs. Journal of Anatomy, 2024: 00, 1–12 To access the full article, click here: The alveolar surface of the lung is lined by an epithelium consisting of type I (AECI) and type II alveolar epithelial cells (AECII). This epithelium

SP-A and SP-D function has been related to innate immunity of the lung based on their binding to sugar residues at pathogen surfaces. Here, we demonstrated that these proteins bind to glycosaminoglycans present at typical cell surfaces like the alveolar epithelial glycocalyx.

In vitro lung research requires appropriate cell culture models that adequately mimic in vivo structure and function. Specifically, these cells are often restricted by terminally altered expression while lacking important alveolar epithelial characteristics.

Registered Report in principle acceptance

The present study provides quantitative data on the differential glycocalyx distribution on AEI and AEII based on cThO2 and demonstrates alveolar glycocalyx shedding in response to HEP or PLY resulting in a structural reduction in both glycosaminoglycan height and density.

We describe ten simple rules to guide participants of relevant training courses in implementing robust research practices in their own projects, once they return to their research group.

Differentiation of THP-1 with 5ng/ml PMA followed by 24h resting period provides a model that morphologically and molecularly resembles macrophages.

Hoffmann, K., Obermayer, B., Hönzke, K., Fatykhova, D., Demir, Z., Löwa, A., Alves, L.G.T., Wyler, E., Lopez-Rodriguez, E., Mieth, M., Baumgardt, M., Hoppe, J., Firsching, T.C., Tönnies, M., Bauer, T.T., Eggeling, S., Tran, H.L., Schneider, P., Neudecker, J., Rückert, J.C., Gruber, A.D., Ochs,